The immunomodulatory activity of ethanol extract of attarasa bark and fruit (Litsea cubeba (lour.) pers.) toward carbon clearance of mice (Mus musculus)

Abstract Background: Attarasa (L.a cubeba (Lour.) Pers.) is a potential Indonesian medicinal plant that used as cold remedy, head ulcers, antimicrobials, antioxidants, and anticancer drugs. Objective: This research was conducted to analyze the immunomodulatory effect of bark (EEKBA) fruit attarasa ethanolic extract (EEBA) by detecting its phagocytosis activity in male mice using carbon clearance method. Method: Total 24 were divided into 6 groups. Extract orally administered mices for 7 days at dose 100 mg/kg BW, 200 400 BW. Imboost® suspension 32.5 BW CMC-Na 1 % positive control, negative control normal On 8th day, 0.1 ml given through intravenous tail injection. The blood samples withdrawn 5, 10, 15, 20 minutes after injection find out absorbance contained measured spectrophotometer then elimination speed, phagocytic index, stimulation index has been calculated Result: EEKBA EEBA induced higher rate compared Phagocytic macrophage with 100, 200, dan 3.429, 3.501, 3.925 consecutively; 4.289, 4.375 4.732 respectively. Stimulation 1.00; 1.20, 1,02; 1,23, 1,13; 1,33. Based on results statistical test, administration stimulate significantly different result group (p < 0.05). Phagocytosis best shown not > Conclusion: have increasing mice. Keywords: immunomodulatory, Litsea cubeba, clearance,